Bioluminescence of the Ca(2+)-binding photoprotein, aequorin, after histidine modification.
نویسندگان
چکیده
Modification studies of the 5 histidine residues in aequorin employing site-directed mutagenesis and diethyl pyrocarbonate suggested that His169 may be the site of binding of molecular oxygen in aequorin. The modification of this residue led to complete loss of activity, whereas modification of the remaining 4 histidine residues yielded mutant aequorins with varying bioluminescence activities.
منابع مشابه
Site-specific mutagenesis of the calcium-binding photoprotein aequorin.
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ورودعنوان ژورنال:
- FEBS letters
دوره 320 3 شماره
صفحات -
تاریخ انتشار 1989